Monday, 25 July 2011

Comparison between HPLC AND HPTLC

Comparison between HPTLC AND HPLC
  • HPLC is not convinent when a sample contain a large number of compounds ,since it requires a large number of mobile phase for their separation, it becomes incontinent and costly
  • In HPLC a solute is eluted out along the solvent and causing interference with the detection of the solute,In HPTLC solvent is evaporated before detection and so there is no interference with solvent
  • HPLC columns are costly .HPTLC plates are cheap when compared to HPLC coumn
  • Very low eluting compounds spoil the costly column in HPLC ,no such danger in HPTLC
  • In HPLC very small sample volume can be applied when compared to HPLC
  • Both in HPLC and HPTLC quantification is possible
  • HPLC can be connected to Mass spectrometer
  • Less time for method development when compared with HPLC
  • HPTLC enables visual observation of separated spots.this permits rapid screening
  • IN HPLC column is used for long time for analogue of many sample because of this sample are retained and accumulated in column and may alter its character.TLC plates are disposible
TAGS-difference between HPLC and HPTLC,lecture notes on HPLC AND HPTLC,advantages of HPLC and HPTLC

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